【摘要】 目的:以支气管上皮细胞和嗜酸粒细胞共培养为实验模型,探讨不同培养方式对IL-6释放的影响.方法:将支气管上皮细胞和嗜酸粒细胞分别单独培养、接触共同培养、分隔共同培养(用分隔膜将两种细胞分隔在上、下两个池中不接触培养,两池中的培养液可以自由通过分隔膜孔)和在Ma-trigel中共同培养.两种细胞在不同培养条件下释放的IL-6应用酶联免疫吸附试验方法定量.结果:支气管上皮细皮与嗜酸粒细胞在同一培养孔中分隔共同培养时其IL-6释放量远高于两种细胞分别在两个培养孔中独自培养后上清液中IL-6之和[(388±40)ng/Lvs(107±10)ng/L,P<0.001].而两种细胞在同一培养孔中(没有隔膜分隔)接触共同培养,其IL-6释放量又远高于其分隔培养[(1270±131)ng/Lvs(388±40)ng/L,P<0.001];将上述两种细胞在Matrigel中共培养,其IL-6释放又显著高于其在非Matrigel中的培养[(2076±165)ng/Lvs(1270±131)ng/L,P<0.01].结论:支气管上皮细胞与嗜酸粒细胞共同培养过程中可受到相互接触和其在培养过程中所释放的某些物质的活化,从而增加多功能细胞因子IL-6的释放.更多还原

【Abstract】 AIM: To investigate the effect of different culture systems on IL-6 release in model of co-culture of human bronchial epithelial cells and eosinophils. METHODS: Human bronchial epithelial cells and eosinophils were cultured or co-cultured in separation, contact and Matrigel system. IL-6 release in different culture systems of bronchial epithelial cells and eosinophils was determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Compared with the sum of IL-6 in culture supernatants of bronchial epithelial cells and eosinophils alone, IL-6 release exhibited a synergistic effects in co-culture of the two kinds of cells in a well separated into up and down chambers by the screen, that could sustain the culture supernatant across [(107±10) ng/L vs (388±40) ng/L, P<0.001]. But IL-6 release in co-culture of the two kinds of cells separated using screen was less than that in their contact co-culture [(388±40) ng/L vs (1270±131) ng/L, P<0.001)]. Co-culture of bronchial epithelial cells and eosinophils in Matrigel system led to asignificant elevation of IL-6 release compared with that in general contact co- culture of the two kinds of cells [(2076±165) ng/L vs (1270±131) ng/L, P<0.01)]. CONCLUSION: Human bronchial epithelial cells and eosinophils can be activated to release IL-6 by contacting each other and mediators that they released in co- culture.更多还原