【摘要】 目的:观察黄芪蛰虫合剂体外抗乙型肝炎病毒(HBV)的作用.方法:以HepG2.2.15细胞为靶细胞,分别加入不同浓度的黄芪蛰虫合剂和α干扰素.采用ELISA法及荧光定量PCR法,测定药物对细胞培养上清HBsAg,HBeAg及细胞外HBVDNA的抑制作用.结果:黄芪蛰虫合剂具有一定程度的抗HBV的作用,其抑制HBsAg,HBeAg及细胞外HBVDNA的50%抑制浓度(IC50)分别为1.35,1.92,2.19g/L.治疗指数(TI)>3.56~5.79.α-IFN也有抑制HBV的作用,但抑制作用较弱.结论:黄芪蛰虫合剂具有一定的体外抗HBV作用.更多还原

【Abstract】 AIM: To evaluate the inhibitive effects of Astragalus and Thoroughfare decoction against hepatitis B virus(HBV) in vitro. METHODS: HepG2.2.15 cells, as target cells, were exposed to different concentrations of Interferon α (IFNα) or Astragalus and Thoroughfare decoction respectively. Real-time PCR was applied to quantify extracellular HBV DNA and enzyme-linked immunosorbent assay (ELISA) was used to assess HBsAg and HBeAg. RESULTS: The 50% inhibitory concentration (IC 50) of Astragalus and Thoroughfare decoction against HBsAg, HBeAg, and extracellular HBV DNA was 1.35, 1.92 and 2.19 g/L respectively, and treatment index (TI)>3.56-5.79. α-IFN had weak inhibitory effects against HBV. CONCLUSION: Astragalus and Thoroughfare decoction can inhibit HBV replication in vitro to some extent.更多还原