【摘要】 目的建立恶性疟原虫顶端膜抗原1(AMA-1)基因转染伯氏疟原虫的模型.方法将含有恶性疟原虫AMA-1基因的重组转染质粒pDB.DTm.DB./AB.AF.DB.用BglI酶切线性化.伯氏疟原虫经体外培养和分离后进行电转化,转化的原虫经药物筛选后进行PCR检测.结果PCR检测显示转染伯氏疟原虫存在恶性疟原虫AMA-1基因.结论成功建立了恶性疟原虫AMA-1基因转染伯氏疟原虫的模型,为进一步研究提供了基础.更多还原

【Abstract】 AIM: To establish the model of Plasmodium berghei (P. berghei) transfected with Plasmodium falciparum (P. falciparum) apical membrane antigen-1 (AMA-1) gene. METHODS: The transfection plasmid pD_B.D_ Tm .D_B./A_B.A_F.D_B. containing P.falciparum AMA-1 was enzymed by BglI. Blood stages of P.berghei were cultured in vitro,and mature schizonts were purified. Parasites transfected by electroporation were injected into tail veins of mice. Transfected parasites were selected by treating mice with pyrimethamine. DNA of transfected,resistant parasites was isolated and analyzed by PCR.RESULTS: The gene encoding P.falciparum AMA-1 was amplified by PCR in the transfected parasites. CONCLUSION: The model of P.berghei transfected with P.falciparum AMA-1 gene was established,which provided a basis for further study.更多还原