【摘要】 目的:探讨脂联素对肿瘤坏死因子(TNF-α)引起的血管内皮细胞炎症反应的影响.方法:体外培养人脐静脉内皮细胞(HUVEC).随机分为对照组,TNF-α刺激组和脂联素+TNF-α刺激组.HUVEC与脂联素联合孵育一段时间后再给予TNF-α刺激与单纯TNF-α刺激做对照,分别采用化学法,放免法及ELISA法检测脂联素对血管内皮细胞NO,iN-OS,ET-1及MCP-1蛋白表达的影响.结果:HUVEC经TNF-α刺激6~12 h后其NO,iNOS,ET-1及MCP-1蛋白的表达(607.7±7.6,42.2±2.2,199.3±11.9,167.7±15.9)与对照组(543.5±2.2,23.8±2.0,148.4±5.3,128.0±8.4)相比明显增强(P<0.05);当HUVEC在TNF-α刺激之前首先与脂联素共同孵育一段时间后,则血管内皮细胞NO,iNOS,ET-1及MCP-1蛋白的表达(565.7±13.0,36.5±2.7,170.8±8.3,142.6±5.6)与单纯给予TNF-α刺激相比明显减弱(P<0.05).结论:脂联素可以通过抑制血管内皮细胞某些炎症因子的表达水平来调节血管内皮细胞的炎症反应,从而发挥其抗炎,抗动脉粥样硬化的作用.更多还原

【Abstract】 AIM: To investigate the influence of adiponectin on the inflammatory reaction in vascular endothelial cells induced by TNF-α.(METHODS:) Human umbilical vein endothelial cells(HUVEC) were cultured in vitro and divided into normal group,TNF-α stimulation group and adiponectin and TNF-α associated stimulation group.HUVEC were preincubated with human recombinant adiponectin for 30 min to 16 h,then exposed to human recombinant TNF-α for 6 to 12 h(adiponectin and TNF-α associated stimulation group),which was then compared with TNF-α stimulation group in the expressions of NO,iNOS,ET-1 and MCP-1.The interaction between adiponectin and HUVEC was estimated by chemical method,radioimmunoassay and ELISA.(RESULTS:) The expressions of NO,iNOS,ET-1 and MCP-1 were increased in HUVEC stimulated by TNF-α for 6 to 12 h(607.7±7.6,(42.2)±2.2,199.3±11.9,167.7±15.9 vs 543.5±2.2,23.8±2.0,148.4±5.3,128.0±8.4;P<(0.05).) Compared with TNF-α stimulation group,the expressions of NO,iNOS,ET-1 and MCP-1 in adiponectin and TNF-α(associated) stimulation group were decreased (565.7±13.0,36.5±(2.7,) 170.8±8.3,142.6±5.6 vs 607.7±7.6,42.2±2.2,(199.3)±11.9,167.7±15.9;P<0.05).CONCLUSION:(Adiponectin) can(modulate) endothelial inflammatory response through effecting the expressions of some inflammatory factors,and adiponectin has(protective) actions against the initiation and (progression) of (atherosclerosis.)更多还原