【摘要】 目的:研究大鼠全脑缺血再灌注损伤后,神经元Na+,K+-ATP酶α亚基三种异构体的变化.方法:采用四血管闭塞法(Pu lsinelli 4-VO)制作全脑缺血15 m in再灌注30m in损伤模型,采用免疫组织化学技术和RT-PCR的方法,检测缺血及再灌注损伤Na+,K+-ATP酶α亚基的改变.结果:缺血及再灌注损伤后,神经元明显水肿;假手术组海马和皮层神经元上主要表达有α1和α3亚基,且α3的含量较α1多,缺血15 min及再灌注30 min后,海马神经元α1和α3亚基的表达明显减少(P<0.05,P<0.01),缺血15 min后,皮层神经元α1和α3亚基的表达无明显改变,而再灌注30 min后表达减少(P<0.05);缺血及再灌注时α1和α3亚基在mRNA水平上的表达与假手术组相比无明显改变.结论:Na+,K+-ATP酶α1和α3亚基可能参与了缺血再灌注脑损伤.更多还原

【Abstract】 AIM: To investigate the change of Na⁺,K⁺-ATPase α isoforms in rat neurons damaged by global cerebral ischemia/reperfusion.METHODS: The globral cerebral ischemia（15 min） in rats were induced by Pulsinelli 4-VO method and the rats were reperfused for 30 min.With RT-PCR and immunohistochemical assay,the expression of the Na⁺,K⁺-ATPase α isoforms was（detected） in rat hippocampal and cortical neurons following（ischemia/reperfusion.） RESULTS: The pyramidal cells during（ischemia/reperfusion） displayed notable edema.The α₁,α₂ or α₃ isoform of Na⁺,K⁺-ATPase distributed in a tissue-specific（fashion） in neurons of hippocampus and cortex,and α₃ isoform was more than α₂ and α₁ isoforms in hippocampus, while there was no significant difference in cortex.The expressions of α₁ and α₃ isoforms in the rats suffering ischemia/reperfusion were significantly decreased in hippocampus （P<0.05 or 0.01）.However,the expressions of α₁ and α₃ in cortex did not change during 15-min ischemia and declined in 30-min reperfusion（P<0.05）.In both ischemia and reperfusion,the mRNA expressions of α₁ and α₃ isoforms at mRNA level had no significant change as compared with sham-operated group.CONCLUSION: The change of the expressions of α₁ and α₃ isoforms may involve in global cerebral ischemia/reperfusioninjury.更多还原