【摘要】 目的:研究白藜芦醇对正常豚鼠心室肌细胞L-型钙电流(ICa-L)的影响.方法:采用全细胞膜片钳技术,记录不同浓度白藜芦醇作用前后对ICa-L的影响.结果:①白藜芦醇呈浓度依赖性阻断ICa-L,但对激活电位、峰电位、反转电位均没有影响;②白藜芦醇使ICa-L稳态激活曲线右移,V1/2由对照的(-18.97±4.08)mV变为(-15.97±4.13)mV(n=7,P=0.02),κ由(3.64±0.56)变为(3.82±0.60)(n=7,P=0.67);③对失活曲线无影响,不改变ICa-L失活特性;④白藜芦醇使ICa-L失活后再恢复时间常数τ缩短,从(98.50±9.00)m s变为(54.53±4.27)ms(n=6,P=0.000).结论:白藜芦醇对ICa-L有阻断作用,主要作用于钙通道激活和恢复过程.更多还原

【Abstract】 AIM: To investigate the effects of resveratrol on the Ltype calcium current I_Ca-L in isolated ventricular myocytes of guinea pig.METHODS: Single ventricular cell was obtained from healthy guinea pig heart using enzymatic dissociation methods.The whole-cell patch clamp was used to record the I_Ca-L following the administration of resveratrol at 1,50,100 μmol/L.RESULTS: ① Resveratrol reduced the I_Ca-L in a concentration-dependent manner,but it had no effect on I-V shape of I_Ca-L.② Resveratrol markedly shifted the steady-state activation curve of I_Ca-L to the right,and V_1/2 value increased from（-18.97±（4.08）） mV in control to（-15.97±4.13） mV in the presence of resveratrol（n=7,P=0.02）,the κ value from（3.64±0.56） to（3.82±0.60）（n=7,P=0.67）.③ Inactivation curve of I_Ca-L was not affected significantly.④ The time constant of reactivation of I_Ca-L was reduced from（98.50±9.00） ms to（54.53±4.27） ms（n=6,P=0.000） by resveratrol.CONCLUSION: Resveratrol inhibited the L-type calcium channel,mainly acting on the activity and recovery processes.更多还原