【摘要】 目的为了满足动物试验的需要,研制GZY型骨质疏松治疗仪,并对其疗效进行评价.方法通过对低强度脉冲磁场非热生物效应机制的分析,结合输出磁场区域均匀度理论计算,提出一种获得匀强磁场的新方法.通过雌性SD大鼠骨质疏松模型来评价仪器的治疗效果.SD大鼠20只随机分为2组(n=10)实验组采用GZY仪器进行治疗,磁感应强度为8×10-4T,10h/d;对照组饲养环境相同,但不暴磁.8wk后,对血清骨钙素、骨密度以及骨形态计量学等参数进行检测.结果①仪器输出匀场的范围要比亥姆赫兹线圈大;②与对照组大鼠相比,实验组骨密度、骨小梁面积百分比、骨小梁宽度、骨小梁数目均有增加[(0.331±0.006vs0.266±0.009)g/cm2,(36.1±1.7vs25.0±1.8)%,(70±5vs48±3)μm,(6.9±0.1vs4.1±0.2)#/mm,P<0.05],骨小梁间隙、血清骨钙素减小[(139±9vs166±11)μm,(1.41±0.10vs2.61±0.09)μg/L,P<0.05].结论GZY型骨质疏松治疗仪能提高实验的整体水平,有益于骨质疏松症的预防和治疗.更多还原

【Abstract】 AIM: To develop a GZY therapeutic instrument for osteoporosis and to evaluate the curative effect. METHODS: By the mechanism analysis of athermal biological effects on low intensity pulsed magnetic fields and the theory of calculating the uniformity of output magnetic fields, a new method of gaining more homogeneous magnetic fields was proposed. To evaluate the curative effect, 20 experimental female SD rat models of osteoporosis were established by ovariectomy. The animal models were randomly divided into 2 groups(n=10, per group): experimental group treated with GZY instrument and control group not exposed to magnetic fields. The two groups were raised in the same conditions. After 8 weeks, the changes of serum osteocalcin, bone mineral density and histomorphometry were examined. RESULTS: ① The output uniform region of GZY instrument was larger than that of Helmholtz coils. ② Compared with those of control group, the values of experimental group such as BMD, trabecular area, trabecular thickness and trabecular number significantly increased[(0.331±0.006 vs 0.266±0.009) g/cm2, (36.1±1.7 vs 25.0±1.8)%, (70±5 vs 48±3) μm, (6.9± 0.1 vs 4.1±0.2) #/mm, P<0.05] but the values of trabecular separation and serum osteocalcin decreased obviously[(139±9 vs 166±11) μm, (1.41±0.10 vs 2.61±0.09) μg/L, P< 0.05] . CONCLUSION: GZY instrument can improve the general level of experiments. It is helpful for the treatment and prevention of osteoporosis.更多还原