【摘要】 目的观察胰岛素对分离培养的大鼠血管平滑肌细胞(vascularsmoothmusclecells,VSMCs)增殖及胶原蛋白合成的影响。方法采用大鼠的腹主动脉血管平滑肌细胞分离培养,应用3H-TdR和3H-脯氨酸掺入方法检测胰岛素对大鼠VSMCs干预后,VSMCs内DNA合成以及胶原蛋白合成。结果胰岛素可促进处于静止状态的大鼠VSMCsDNA及胶原蛋白的合成,并呈现明显的浓度依赖关系,在40nmol/L的浓度时DNA及胶原蛋白的合成达到高峰,DNA及胶原蛋白分别处于36h和48h时合成最为显著。结论胰岛素可明显促进培养的VSMCs增殖及胶原蛋白的合成。更多还原

【Abstract】 Objective To understand the role of insulin in the cytobiology of blood vessels.Methods The vascular smooth muscle cells(VSMCs)from SD rat were cultured and respectively treated with 1,10,20,30,40 nmol/L insulin for 24 h or 30 nmol/L insulin for 12,24,36,48 h,or with DMEM solution containing 10% FBS as blank control.DNA synthesis and collagen protein synthesis of rat VSMCs were detected by 3H-TdR incorporation and 3H-proline incorporation.Results Insulin could significantly promote DNA synthesis and collagen protein synthesis of quiescent rat VSMCs in a dose-dependent manner.DNA synthesis and collagen protein synthesis reached the peak when insulin concentration was 40 nmol/L,and showed significant difference at 36 h and 48 h with that of the blank control.Conclusion Insulin could promote proliferation and collagen protein synthesis of cultured rat VSMCs.更多还原