【摘要】 目的观察APE1siRNA表达载体pSilenceAPE1对骨肉瘤诱导血管内皮细胞迁徙的抑制作用及其与ES的协同作用。方法pSilenceAPE1转染骨肉瘤细胞9901和HOS;骨肉瘤和内皮细胞Transwell双室培养,计数迁移到内室膜背面的内皮细胞数,观察pSilenceAPE1及其联合ES对骨肉瘤细胞诱导内皮细胞迁徙的影响。结果ES低剂量组(350ng/ml)和高剂量组(700ng/ml)与正常对照组有明显差异(P<0·01)。2μgpSilenceAPE1和3μgpSilenceAPE1较单独脂质体组有显著性差异(P<0·01)。2μgpSilenceAPE1和ES低剂量(350ng/ml)联合组抑制内皮细胞迁徙的作用显著高于单独对照组(P<0·01)。结论pSilenceAPE1可显著抑制骨肉瘤细胞诱导的血管内皮细胞迁徙,并且可能与ES具有协同抗血管生成的作用。更多还原

【Abstract】 Objective To determine the inhibitory effect of APE1 siRNA expression vector pSilence APE1, and the possible synergetic role of pSilence APE1 and endostatin on endothelial cell migration induced by osteosarcoma cell 9901 and HOS . Methods The osteosarcoma cells and endothelial cells were co-cultured with transwell model, and the cell number through the inner membrane was counted to evaluate the inhibitory effect of endothelial cell migration by pSilence APE1 and its combination with endostatin. Results Both low dose (350 ng/ml) and high dose (700 ng/ml) of endostatin showed significant inhibition to endothelial cell migration induced by osteosarcoma cell, while the high dose showed much stronger inhibition than the low dose (P<0.01), such as the 2 μg pSilence APE1 and 3 μg pSilence APE1(P<0.01). Moreover, the combination of 2 μg pSilence APE1 with low dose endostatin (350 ng/ml) showed markedly stronger inhibition to endothelial migration than either of 2 μg pSilence APE1 or low dose endostatin (P<0.01). Conclusion pSilence APE1 can inhibit the endothelial cell migration induced by the osteosarcoma, and it may have a potential synergistic role with endostatin in antiangiogenesis.更多还原