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TaNHX₂基因植物表达载体的构建及在拟南芥中的功能分析

Construction of Phyto-expression Vector and Functions in Arabidopsis thaliana of TaNHX₂ Gene

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【Author】 YU Jia-ning~1,YUAN Jiang-feng~2,YANG Jian-xiong~(2*),ZHAO Yong-mei~3,WANG Zhe-zhi~2,ZHANG Zhi-qi~2(1 College of Life Science,Shaanxi Normal University,Xi’an 710062,China;2 Key Laboratory of Medicinal Plant Resources and Nature Pharmaceutical Chemistry(Ministry of Education),Shaanxi Normal University,Xi’an 710062,China;3 Department of Life Sciences,Xi’an University of Arts and Science,Xi’an 710065,China)

【机构】陕西师范大学生命科学学院；陕西师范大学药用植物资源与天然药物化学教育部重点实验室；西安文理学院生命科学系；陕西师范大学药用植物资源与天然药物化学教育部重点实验室西安710062；西安710062；西安710065；

【摘要】将TaNHX2基因重组于质粒pBIN438的CaMV 35S启动子下游,构建含TaNHX2基因的植物双元表达载体pBIN438-TaNHX2。采用根癌农杆菌介导的真空渗透法转化拟南芥,得到T0代转基因拟南芥种子。经含Kan的平板筛选及PCR鉴定,获得54株阳性植株,选取生长一致的转基因阳性植株进行耐盐、耐旱分析,结果表明TaNHX2能够提高转基因植株的耐盐性和耐旱性。更多还原

【Abstract】 TaNHX₂ was recombined to the downstream of CaMV35S promotor in plasmid pBIN438 to form binary phyto-expression vector pBIN438-TaNHX₂.pBIN438-TaNHX₂ was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated vacuum filtration and then T₀ transgenic seeds of Arabidopsis thaliana were obtained.54 positive plants were obtained by kanamycin plate screening and PCR identification and uniformly-growing ones of the plants were taken to study their salt and drought tolerances and TaNHX₂ was found to be capable of increasing the salt and drought tolerances of the transgenic plants.更多还原