【摘要】 利用形态学、细胞学以及SSR标记技术对从硬簇麦和Am 3的杂种后代中选育的种质系‘山农030713’进行了鉴定,结果表明:种质系‘山农030713’大田生长整齐一致,农艺性状较好,且对白粉病免疫;其根尖细胞染色体数目为2n=42,花粉母细胞减数分裂中期Ⅰ(PM C MⅠ)染色体构型为2n=21Ⅱ;它与普通小麦的杂种F1PM C MⅠ多数细胞中形成21个二价体,且常有四价体出现,可能伴有染色体的结构变异;SSR分析证明‘山农030713’基本染色体组成为AABBDD,引物X gwm 99-1A在‘山农030713’中扩增出簇毛麦的特异带,表明‘山农030713’中有来自于簇毛麦的遗传物质,此特异带可作为识别‘山农030713’的SSR标记.综合形态学、细胞学和SSR分析结果推测,‘山农030713’可能是一个小麦-簇毛麦易位系.更多还原

【Abstract】 SSR and morphological and cytological selection were adopted to identify the germplasm line ’Shannong 030713’ bred from the progenies of Am3×Triticum aestivum-Haynaldia villosum amphidiploid,and the results showed that in the field,’Shannong 030713’ grew evenly presenting favorable agronomic traits and it was immune to powdery mildew;the chromosomes numbered 42(2n=42) in its root tip-cells and the chromosome configuration was 2n=21Ⅱ at the PMC MⅠ;At PMC MⅠ of its hybrids with common wheat,twenty-one bivalents appeared in most cases but qudravialents frequently occurred,and this indicated that the variation in chromosome structure might take place.SSR analysis of ’Shannong 030713’ proved that the gnomic composition of ’Shannong 030713’ was AABBDD and a band specific to H.villosum were amplified from with the primer Xgwm99-1;this indicated that ’Shannong 030713’ contained genetic materials from H.villosum and the band could be used as a SSR marker to identify ’Shannong 030713’.It can be deduced from these results in morphology and cytology and SSR analysis that ’Shannong 030713’ is probably a T.aestivum-H.villosum translocation line.更多还原