【摘要】 用灵敏度较高的RT-PCR检测培养4周的大豆幼苗的5个不同组织:嫩叶、老叶、茎、离层和根,测得脱落纤维素酶基因的表达量互不相同,离层中表达量最高,茎中表达量最低。选取表达量最高的离层作为逆境处理材料,分别用高温(46℃)、干旱、盐(200 mm o l/L N aC l)处理不同时间后,检测脱落纤维素酶基因的时间表达模式。结果表明:3种逆境条件下,脱落纤维素酶基因的时间表达模式各不相同,但总的来说,高温能抑制脱落纤维素酶基因的表达,干旱和盐都能促进脱落纤维素酶基因的表达。更多还原

【Abstract】 High-sensitivity RT-PCR was adopted to examine five different tissues,young leaves,old leaves,stems and abscission layers and roots,of soybean seedlings that had been cultured for four weeks,and they were tested to show different expressions of soybean abscission cellulose gene,the highest expression amount appearing in abscission layers,and the lowest expression amount occurring in stems.The abscission layers with highest expression amount was chosen as the material to be treated in adverse environments and then were treated separately at 46℃,under drought,and with salt(200 mol/L NaCl) for different period of time to test their temporal patterns of soybean abscission cellulose gene.The results showed that under the three kinds of adverse environments,the temporal expression patterns differed,but as a whole,high temperature could inhibit the expression of soybean abscission cellulose gene,and drought and salt could boost the expression of soybean abscission cellulose gene.更多还原