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蛹虫草发酵液抗菌活性初步研究

Preliminary Study of the Anti-microbial Activities of Fermentation Broth of Cordyceps militaris

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【作者】 秦建春李晓明张鞍灵董艳红高锦明

【Author】 QIN Jian-chun,LI Xiao-ming,ZHANG An-ling,DONG Yan-hong,GAO Jin-ming~*(College of Life Science,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China)

【机构】 西北农林科技大学生命科学学院西北农林科技大学生命科学学院 陕西杨陵712100陕西杨陵712100

【摘要】 对蛹虫草摇瓶发酵的乙酸乙酯和正丁醇萃取物进行了抗菌实验.结果表明,乙酸乙酯萃取物对细菌中的金黄色葡萄球菌、蜡状芽孢杆菌、变形杆菌、巨大芽孢杆菌、北京棒状杆菌和霉菌中的绿色木霉、黄曲霉有明显的抑菌作用;正丁醇萃取物对细菌中的马铃薯芽孢杆菌、变形杆菌、枯草芽孢杆菌、灵杆菌和霉菌中的绿色木霉以及黄曲霉有明显的抑菌作用,且提取物的抑菌作用随浓度增大而增强,而水相则没有抑菌活性.蛹虫草发酵液中具有抗菌活性物质.

【Abstract】 The broth of Cordyceps militaris fermentation in an oscillating manner were extracted with EtOAa and n-BuOH and the anti-microbial activity of the extracts were tested.The results showed that the extract by EtOAc presented a significant effect on Staphylococcus aureau、Bacillus cereus、Bacillus megaterium、Proteus species,Corynebacterium pekinense、Trichoderma viride and Aspergillus flavus all of which belonged to bacteria;the extract by n-BuOH presented significant inhibitory effect on Bacillus mesentericus、Bacillus subtilis、Proteus species、Bacterium prodigious,Trichoderma viride and Aspergillus flavus all of which belonged to bacterium;The inhibitory effect become stronger and stronger with the extract concentration increaseing and the water phase of the extract did not present any antimicrobial effects.As a result,the broth of Cordyceps militaris fermentation contained antimicrobial active substances.

【基金】 国家自然科学基金项目(30370019);国家“十五”攻关项目(2002BA516A04)
  • 【文献出处】 西北植物学报 ,Acta Botanica Boreali-Occidentalia Sinica , 编辑部邮箱 ,2006年02期
  • 【分类号】R285.5
  • 【被引频次】61
  • 【下载频次】677
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