【摘要】 利用高效液相色谱法建立了青海秦艽的色谱指纹图谱.色谱条件:K rom as il C18柱(250 mm×4.6 mm×5μm),甲醇∶磷酸水(0.04%)梯度洗脱,流速为1 mL/m in,检测波长222 nm.通过比较发现秦艽样品的13个主要共有峰,可作为鉴别秦艽药材的主要依据.对12批秦艽药材进行了相似度计算和聚类分析,结果表明,所建立的秦艽指纹图谱可用于秦艽药材的真伪鉴别和质量评价.更多还原

【Abstract】 The fingerprinting of Gentiana straminea Maxim.from Qinghai province was established by HPLC.The HPLC conditions were that Kromasil C₁₈ column（250 mm×4.6 mm×5 μm） and CH₃OH-0.04% H₃PO₄ gradient were chosen and the flow speed the test wavelength were set at 1 mL/min and 222 nm,respectively.Thirteen common peaks were found by comparison capable of being used as the major determinants identifying G.straminea.The similarity calculation and cluster analysis of twelve batches of G.straminea were conducted indicating that the established fingerprinting of G.straminea was suitable for telling genuine G.straminea from bogus ones and evaluating their quality of G.straminea.更多还原