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硬粒小麦—节节麦人工合成种HMW-GS组成及1.5+10亚基的遗传分析

HMW-GS Composition and 1.5+10 Subunit Genetic Analysis of Wheat Syndeme Developed with Durum Wheat and Aegilops tauschii

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【作者】 王世红张怀刚周宽基袁明璐刘宝龙张梅妞张波

【Author】 WANG Shi-hong~(1,2,3),ZHANG Huai-gang~(1*),ZHOU Kuan-ji~2,YUAN Ming-lu~2, LIU Bao-long~(1,3),ZHANG Mei-niu~1,ZHANG Bo~(1,3)(1 Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xi’ning 810001,China;2 Crop Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;3 Graduate School,Chinese Academy of Sciences,Beijing 100039,China)

【机构】 中国科学院西北高原生物研究所甘肃省农业科学院粮食作物研究所中国科学院西北高原生物研究所 西宁810001兰州730070中国科学院研究生院北京100039西宁810001

【摘要】 对C IMM YT的99份硬粒小麦—节节麦人工合成种(简称合成种)的HMW-G S组成分析发现,G lu-B 1和G lu-D 1位点的变异类型比普通小麦丰富,分别有9种和12种亚基类型;筛选出含有比5+10亚基更优质的1.5+10和5+12亚基的合成种分别有8份和1份;含有优质亚基1.5+10的合成种与普通小麦杂交结实正常;对2个合成种与2个普通小麦品种的8个正反交组合F1种子电泳发现,优质亚基1.5+10在F1代能正常表达,双亲所有亚基在F1代都得到表达,表现共显性遗传.本研究为优质亚基1.5+10和5+12转育到普通小麦中奠定了基础.

【Abstract】 The HMW-GS compositions of 99 materials of the syndeme developed by CIMMYT with durum wheat and Aegilops tauschii(Syndeme in short) were explored and then Glu-B1 and Glu-D1 were found to vary in their types more rechly than those in common wheat,and contained 9 and 12 subunit types,respectively.There are 8 materials and 1 material of the syndeme screened to contain subunit 1.5+10 and subunit 5+12,which were better in quality than subunit 5+10,respectively.The syndeme materials containing the quality subunits were used to cross common wheat and their seed formation took place normally.The electrophoresis of the F1 seeds of the reciprocal and direct crosses of two syndeme materials and 8 common wheat varieties indicated that subunit 1.5+10,a quality subunit,could normally express in F1 and all the subunits of the male and female parents could express in F1,and thus subunit 1.5+10 presented a co-dominant inheritance.This study laid a foundation for transforming quality subunit 1.5+10 and 5+12 into common wheat.

【关键词】 节节麦人工合成种HMW-GS遗传分析
【Key words】 Aegilops tauschiisyndemeHMW-GSgenetic analysis
【基金】 中国科学院知识创新工程项目(KSCX2-SW-304);甘肃省高新技术攻关项目(GSK05-004)
  • 【文献出处】 西北植物学报 ,Acta Botanica Boreali-Occidentalia Sinica , 编辑部邮箱 ,2006年01期
  • 【分类号】S512.19
  • 【被引频次】4
  • 【下载频次】102
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