【摘要】 [目的]探讨蝎毒(BMK)对人乳腺癌MCF-7/ADM细胞多药耐药的逆转及谷胱甘肽S转移酶(GST)活性的影响。[方法]实验分两组:MCF-7/ADM耐药细胞组;MCF-7/ADM+蝎毒组。采用MTT法测定蝎毒的细胞毒性和抗药性逆转,流式细胞术测定蝎毒对耐药细胞凋亡百分率的影响,紫外分光光度术测定蝎毒对谷胱甘肽S-转移酶(GSTs)活性的影响。[结果]与MCF-7/ADM耐药细胞组相比:①非细胞毒性剂量(3.0μg/mL)蝎毒能显著降低MCF-7/ADM的IC50(P<0.01),逆转倍数为1.52倍。②蝎毒(3.0μg/mL)显著增强对耐药细胞的凋亡诱导作用,凋亡率由(8.9±0.01)%上升为(12.6±0.21)%(P<0.01)。③蝎毒(3.0μg/mL)显著降低耐药细胞内谷胱甘肽S转移酶的活性(P<0.01)。[结论]蝎毒能够部分逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性,增加ADM对肿瘤细胞的凋亡诱导作用,其逆转机制与降低耐药细胞内GST酶活性有关。更多还原

【Abstract】 [Objective] To study the effect of inducing apoptosis and resistance reversing on BMK combined with ADM in human breast cancer MCF-7/ADM cell line.[Methods] The major groups were divided as follows:MCF-7/ADM cell lines;MCF-7/ADM cell lines with BMK.The cytotoxicity and the reversal of resistance of BMK were assessed by MTT assay.The apoptosis percentage were determined by the flow cytometry.The glutathione s-transferase activity were determined by the UV-spectrophotometry.[Results]Compared with MCF-7/ADM cell lines:①The non-cytotoxic dose of BMK（3 μg/mL） significantly decreased the IC₅₀ value of ADM（P<0.01）.Its reversal folds is 1.52.②BMK（3 μg/mL） increased the apoptosis inducing effect of ADM to MCF-7/ADM cells,which of apoptosis percentage was increased from （8.9±0.01）% to（12.6±0.21）%（P<0.01）.③BMK（3μg/mL） could significantly decrease glutathione s-transferase activity in MCF-7/ADM cells（P<0.01）.[Conclusion] BMK could partially reverse resistance to ADM and increase the apoptosis in human breast cancer MCF-7/ADM cell line,which is related to decreasing glutathione s-transferase activity.更多还原