【摘要】 研究了应用斑点酶联免疫吸附试验(Dot-Enzym e L inked Immunosorbent Assay,Dot-ELISA)技术检测副溶血弧菌Vibrio parahaem olyticus的方法。根据棋盘试验,确定副溶血弧菌免疫血清最佳工作浓度为1∶200,酶标抗体最佳工作浓度为1∶100,以出现明显清晰斑点者判定为阳性。用该方法检测时,副溶血弧菌呈阳性,哈维氏弧菌V.harveyi、嗜水气单胞菌Aerom onas hydrophila、河流弧菌Vf.luvialis biotype、溶藻弧菌V.alginolyticus、大肠杆菌Escherichia coli均呈阴性。斑点ELISA方法不仅具有快速、经济的特点,而且可用肉眼直接判定结果,适合在基层单位应用推广。更多还原

【Abstract】 A Dot-Enzyme Linked Immunosorbent Assay(Dot-ELISA) was established for detection of Vibrio parahaemolyticus.The working concentration of serum samples was 1∶200 dilution and that of the enzyme-labeled goat anti-rabbit IgG was 1∶100 dilution by chessboard assay.The samples with clear and obvious dots were judged as positive reaction.V.parahaemolyticus was positive in the Dot-ELISA,while V.harveyi,Aeromonas hydrophila,V.fluvialis biotype,V.alginolyticus and Escherichia coli were negative in the assay.Dot-ELISA was characterized by rapid reaction,economical feasibility,and observable results and widely adopted.更多还原