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鼠疫放射免疫沉淀试验的应用分析

An Analysis of Application of Plague Radioimmunoprecipitation

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【作者】 张曼碧刘小华冯志勇杨华源梁秋光杨柳潘珠黄济英

【Author】 ZHANG Man-bi~1,LIU Xiao-hua~1,FENG Zhi-yong~2,YANG Hua-yuan~1,LIANG Qiu-guang~1,YANG Liu~1,PAN Zhu~1,HUANG Ji-ying~1 (1.Zhanjiang Institute of Plague Prevention and Control of Guangdong Province,Zhanjiang City,Guangdong 524037,China;2.Phytoprotection Institute of Guangdong Provincial Agricultural Scientific Academy)

【机构】 广东省湛江鼠疫防治研究所广东省农业科学院植物保护研究所广东省湛江鼠疫防治研究所 广东湛江524037广东湛江524037

【摘要】 目的进一步了解鼠疫放射免疫沉淀试验(R IP)的敏感性、特异性和现场应用的实用性。方法被检血清经56℃30m in灭活后按R IP试剂盒说明进行(设F1抗原抑制、阴性血清、空白对照)。结果鼠疫菌单独和混合其它菌抗血清全部阳性;伪结核、小肠结肠炎、中间型耶氏菌等单独抗血清和现场鼠类血清、人血清、正常小白鼠血清、IHA非特异性鼠类血清全部阴性。结论R IP的敏感性高、特异性好,现场应用未检测到鼠疫抗体,说明目前广东省鼠疫处于静息期。

【Abstract】 Objective The sensitivity and specificity of RIP(radioimmunoprecipitation) and its practicality were further studied.Methods The sera detected were inactivated for 30min at 56℃ and tested according to the requirement of directions of RIP kit(three procedures: F1Ag inhibition,negative sera and blank control).Results Yersinia pestis sera and mixed bacteria(three groups:Yersinia pestis plus Y.pseudotuberculosis;Y.pestis plus Y.enterocolitica and Y.pestis plus Y.intermedia) sera were all positive separately;sera of Y.pseudotuberculosis,Y.enterocolitica and Y.intermedia,rodents sera,human sera,normal mice’ sera and rodents’ sera of non-specificity in IHA were all negative respectively.Conclusions RIP has a high sensitivity and good specificity and plague antibody has not been detected in the field.It suggests that plague in the focus be in silent period.

【基金】 广东省第二批科学事业费计划项目(2004A20403003)
  • 【文献出处】 地方病通报 ,Endemic Diseases Bulletin , 编辑部邮箱 ,2006年01期
  • 【分类号】R817.4
  • 【被引频次】1
  • 【下载频次】69
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