【摘要】 目的评价胶体金免疫层析法(G ICA)对鼠疫腐败材料检测效果。方法将感染的动物脏器,分别置于室温(25℃)和37℃下,在保存的不同时期,用胶体金试纸条进行检测,细菌培养和反向血凝(R IHA)同步检测。结果感染材料,保存300d后,G ICA、R IHA检测均为阳性,20天后细菌培养为阴性;正常材料,保存300d后R IHA检测为阳性,而G ICA检测为阴性。结论对鼠疫腐败材料的检测G ICA优于R IHA和细菌培养,可广泛应用于现场材料的检测。更多还原

【Abstract】 Objective To observe the effectiveness evaluation of testing rotten viscera samples with GICA.Methods We kept the infectious and normal viscera samples under ambient temperature and 37℃,and tested them with GICA,RIHA and germiculture at different keeping time.Results The infectious samples were positive with GICA and RIHA after 300 days,but they were negative with germiculture after 20 days.The normal samples were negative with GICA but positive with RIHA after 300 days.Conclusions GICA is better than RIHA and germiculture in testing rotten samples,and it can be wildly used in testing field samples.更多还原