【摘要】 目的:从海洋微生物中寻找天然活性物质。方法:利用稻瘟霉作为菌株筛选模型,对筛选出来的活性菌株F8712发酵液的醋酸乙酯提取物进行化合物分离;通过1HNMR1、3CNMR和ESI-MS对化合物进行结构鉴定。结果和讨论:鉴定了6个环二肽类化合物,它们分别为环[Ala-Leu](Ⅰ),环[Ala-Ile](Ⅱ),环[Val-Pro](Ⅲ),环[Leu-Pro](Ⅳ),环[Ile-Pro](Ⅴ)和环[Leu-Val](Ⅵ)。这些环二肽都没有抗稻瘟霉活性,化合物Ⅴ对鳗弧菌(Vibrio anguillarm)具有较强的抑制能力,其MIC为0.07μg/ml。更多还原

【Abstract】 Objective:To search for bioactive compounds from marine microbes.Methods: Pyricularia oryzae was used to screen microbes.Compounds extracted with EtOAc from the fermentation broth of F8712 strain（an active microbe screened out） were separated.The chemical structures of the separated compounds were identified by ¹HNMR,¹³CNMR and ESI-MS techniques.Results:Six cyclic dipeptides,cyclo（Ala-Leu）,cyclo（Ala-Ile）,cyclo（Ile-Pro）,cyclo（Val-Pro）,cyclo（Leu-Pro） and cyclo（Leu-Val）,were obtained.Conclusion: None of the 6 cyclodipeptides has any anti-Pyricularia oryzae activities.Compound Ⅴ has potent inhibitory effects on Vibrio anguillarm with a minimum inhibitory concentration of 0.07 μg/ml.更多还原