【摘要】 目的研究大鼠蛛网膜颗粒在颅内的分布,为实验取材及其相关研究奠定基础。通过动物实验分析蛛网膜下腔出血(SAH)后的蛛网膜颗粒细胞凋亡。方法亚甲蓝组大鼠小脑延髓池内注入亚甲蓝,亚甲蓝广泛分布于蛛网膜下腔,蛛网膜颗粒摄取亚甲蓝呈现兰色,记数蛛网膜颗粒的数目及分布。SAH组分为A、B、C、D、E组,A组为对照组,小脑延髓池内注入生理盐水,B、C、D、E组为实验组,小脑延髓池注入自体动脉血溶解物,于10 d处死对照组(A组)大鼠,分别于注血后1 d(B组)、4 d(C组)、7 d(D组)、10 d(E组)处死实验组大鼠。取硬脑膜窦进行石蜡包埋、切片,采用TUNEL法检测蛛网膜颗粒的细胞凋亡。结果90.7%的蛛网膜颗粒分布于横窦内,6.4%分布于矢状窦内,2.9%分布于硬脑膜。A组蛛网膜颗粒中偶见细胞凋亡,B、C、D、E 4组蛛网膜颗粒中凋亡细胞逐渐增多。蛛网膜细胞、内皮细胞及纤维细胞三类细胞均有凋亡现象。结论大鼠的蛛网膜颗粒主要分布在横窦内。SAH后存在过度的炎症反应过程,使蛛网膜颗粒出现过度的细胞凋亡。更多还原

【Abstract】 Objective To find the location of arachnoid granulations in cranial cavity of rats and to analyze the apoptosis of arachnoid granulations cells by the animal experiments model of the subarachnoid hemorrhage(SAH).Methods The methylene blue was injected into rats’ cerebellomedullary cistern and diffused in the subarachnoid space.The number and location of the blue arachnoid granulations were detected.The rats were divided into 5 groups: group A,B,C,D and E.In group A as a control,the saline was injected into the cerebellomedullary cistern of the rats and the rats were killed after 10 days.In group B,C,D and E,the arterial hemolysate of the rats was injected into the cerebellomedullary cistern and the rats were killed after 1day(group B),4 days(group C),7 days(group D) and 10 days(group E) respectively.The parts of dural sinus were cut off and embedded with paraffin and section.The apoptosis of arachnoid granulations cells was tested by the TUNEL method.Results The 90.7% of arachnoid granulations was located in the lateral sinus,6.4% in the sagittal sinus and 2.9% in the dura.The apoptosis of arachnoid granulations cells could be detected in the rats of group A and the rates of apoptosis were gradually increased in the rats of group B,C,D and E.The apoptosis of arachnoid cells,endothelial cells and fibrocytes cells could be detected in the rats of group B,C,D and E.Conclusion Most of the rat arachnoid granulations in rats are located in lateral sinus.There is excessive inflammatory reaction after SAH,which causes the excessive apoptosis of arachnoid granulations.更多还原