【摘要】 目的大量研究表明内源性结缔组织生长因子(CTGF)在肾小管间质纤维化(TIF)进程中发挥了重要作用,为进一步阐明外源性CTGF的作用机制,该实验探讨了重组人结缔组织生长因子(rhCTGF)刺激对人肾小管上皮细胞(HK2)转分化和胶原合成的作用。方法rhCTGF 5 ng/mL刺激HK2细胞,用倒置显微镜观察细胞形态学变化,同时在刺激后0,3,6,12,24,48 h各点收集细胞,RT-PCR检测上皮细胞钙粘蛋白(E-cadherin)、α-平滑肌肌动蛋白(α-SMA)、胶原Iα1(Col Iα1)和胶原IVα1(Col IVα1)mRNA表达变化。结果rhCTGF 5 ng/mL刺激使HK2细胞形态由椭圆形转为梭形,下调E-cadherin mRNA表达,上调-αSMA mRNA表达,但对Col Iα1和Col IVα1mRNA表达没有影响。结论外源性CTGF能导致HK2细胞转分化,但不能促进其胶原合成。更多还原

【Abstract】 Objective To investigate the effects of recombinal human connective tissue growth factor（rhCTGF） stimulation on epithelial-myofibroblast transdifferentiation（EMT） and collagen-synthesis in human renal tubular epithelial cell line（HK2） in vitro.Methods The cultured HK2 cells were stimulated with rhCTGF of 5 ng/mL.The morphological changes were observed under an inverted microscope.The cells were collected at 0,3,6,12,24 and 48 hrs after rhCTGF stimulation.The expression of E-cadherin,α-smooth muscle actin（α-SMA）,collagen Iα₁（Col Iα₁） and collagen IVα₁（Col IVα₁） mRNAs were detected by RT-PCR.Results rhCTGF stimulation changed the HK2 cell appearance from oval to fusiform,down-regulated the E-cadherin mRNA expression and up-regulated α-SMA mRNA expression,but had no effects the Col Iα₁ and Col IVα₁ mRNA expression. Conclusions Exogenous CTGF can mediate the EMT but has no collagen-synthesis effects on HK2 cells.更多还原