【摘要】 以风箱果幼苗顶芽为外植体,诱导不定芽,通过试管外生根技术建立植株再生体系。结果表明：以MS+BA1．0 mg·L^-1+NAA1．0 mg·L^-1为诱导培养基,MS+BA0．8 mg·L^-1+NAA0．4 mg·L^-1为增殖培养基,MS+IBA0．5 mg·L^-1+GA₃0．3 mg·L^-1为壮苗伸长培养基,效果较好；试管外生根以100 mg·L^-1NAA处理30 min效果最佳,生根成活率为93．1%。更多还原

【Abstract】 In vitro adventitious shoot was induced from apical bud of Physocarpus amurensis seedlings,and establishment of pla- ntlet regeneration system was studied by ex vitro rooting of the in vitro regenerated microshoots.Results showed that the op- timum culture media were obtained as MS+BA 1.0 mg·L^-1+NAA 1.0mg·L^-1for apical bud germination,MS+BA 0.8mg·L^-1+NAA 0.4 mg·L^-1 for shoot proliferation,and MS+IBA 0.5 mg·L^-1+GA₃ 0.3mg·L^-1for shoot elon- gation and strengthening.The best treatment for ex vitro rooting was 100 mg·L^-1NAA soaked for 30 min,and the rooting and survival rate reached 93.1%.更多还原