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松苗猝倒病不同病原物的致病性差异及其生防细菌的筛选

Difference in Pathogenicity of Damping-off Pathogens for Pine Seedlings and Screening of Biocontrol Bacteria

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【Author】 Chen Jingyuan(Nanjing Forestry University, Nanjing 210037, P. R. China); Xu Hongmei, Cai Sanshan(Hubei Forestry Academy); Huo Xianqi(Huazhong Agricultural University); Yang Daigui(Pengchang Forest Farm of Jingmen, Hubei Province); Hou Mingsheng(Huazhong Agricultural University); Ji Baohong(Nanjing Forestry University)/

【机构】南京林业大学；湖北省林业科学研究院；华中农业大学；湖北省荆门市彭场林场；南京林业大学南京； 210037；

【摘要】从3个湿地松苗圃土壤中分离出了66株松苗猝倒病的致病真菌,63个菌株为镰孢属真菌,2个为链格孢菌,1个为丝核菌属真菌。对66个真菌菌株进行松苗猝倒病的致病性测定表明:12个真菌菌株为无致病性或弱致病性真菌,17个镰孢属菌株为强致病性真菌,其余37个为中等程度致病性真菌。从湿地松幼苗根系分离到了细菌菌株320株,平皿拮抗测定表明其中的10个菌株对松苗猝倒病病原真菌有强拮抗作用,对松苗猝倒病有较大的生防潜力。16SrDNA序列分析表明这10个生防菌株的分类地位分别是:7株为洋葱伯克霍尔德菌,2株为枯草芽孢杆菌,1株为待定种。更多还原

【Abstract】 66 isolates of pathogen fungi were got and identified from the soil in three pine nurseries, among which 63 isolates belong to Fusarium spp., 2 Alternaria alternata and 1 Rhizoctonia sp. Pot-testing resulted in 12 isolates with low-pathogenicity, 17 with high-pathogenicity and 37 with medium-pathogenicity. 320 strains of bacteria were isolated from rhizosphere soil for pine seedlings. According to in vitro assay, 10 strains had strong antagonism against the indicator pathogen. 16S ribosomal DNA sequence analysis showed that among 10 potential biocontrol strains, 7 strains were Burkholderia cepacia, 2 Bacillus subtilis and 1 unidentified strain.更多还原

【关键词】松苗；猝倒病；致病性；生防细菌； 16SrDNA序列分析；
【Key words】 Pine seedlings； Damping-off； Pathogenicity； Biocontrol bacteria； 16S ribosomal DNA sequence analysis；

【基金】国家林业局“948”项目(2003-4-39)

【文献出处】东北林业大学学报 ,Journal of Northeast Forestry University , 编辑部邮箱 ,2006年02期

【分类号】S763.7
【被引频次】13
【下载频次】225

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