【摘要】 [目的]探讨川芎嗪(TMP)对人乳腺癌MCF-7/ADM细胞的耐药逆转及其对该细胞P-糖蛋白(P-gp)表达的影响.[方法]MTT法测定细胞的药敏性和抗药性逆转,流式细胞术检测耐药细胞P-糖蛋白的表达.[结果]非细胞毒性剂量(320μg/m l)和低毒剂量(1 250μg/m l)的川芎嗪均能显著降低MCF-7/ADM细胞的IC50(P<0.01),逆转倍数分别为2.14和2.80倍;并使该细胞P-gp的表达率由(90.60±0.40)%分别降低至(69.10±1.65)%和(60.30±1.25)%.[结论]川芎嗪可部分逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性,其逆转机制与抑制该细胞P-gp的表达有关.更多还原

【Abstract】 [Objective]To investigate the effect of TMP on multidrug resistance reversal and P-glycoprotein（P-gp） expression in human breast cancer MCF-7/ ADM cell line.[Methods] The sensitivity of MCF-7/ADM cells and the reversal of resistance were assessed by MTT assay.P-gp expression was determined by flow cytometry.[Results] The non-cytotoxic dose（320g/ml） and low-cytotoxic dose（320g/ml） of TMP decreased significantly the IC₅₀ value of ADM to MCF-7/ADM cells（P<0.01）,and reversed the resistance by 2.14 folds,2.80 folds respectively compared with the IC₅₀ value in MCF-7/ADM and reduced P-gp expression from（90.60±0.40）% to（69.10±1.65）% and（60.30±1.25）% respectively.[Conclusion]: TMP could partially reverse the resistance to ADM in human breast cancer MCF-7/ ADM cell line,which may be related to inhibiting P-gp expression in MCF-7/ADM cells.更多还原