【摘要】 目的通过研究integrinα5β1(整合素α5β1)在卵巢上皮性肿瘤中的表达,探讨其与卵巢癌淋巴结转移的关系。方法用免疫组化ElivisionTM法检测80例卵巢上皮性肿瘤中integrinα5β1的表达;运用图像分析系统进行定量检测。结果①定性及半定量结果integrinα5β1的表达按良性、交界性及恶性的顺序呈递减趋势,组间均有显著差异(P<0.01);integrinα5β1淋巴结转移组与未转移组间表达有显著差异(P<0.01)。②定量检测结果integrinα5β1表达的阳性细胞面积按良性、交界性、恶性的顺序呈递减趋势,灰度呈递增趋势。结论integrinα5β1的表达与肿瘤的侵袭、转移有关;integrinα5β1表达下降或缺失,提示肿瘤进展,恶性度高,预后不良;计算机图像分析系统为卵巢上皮性肿瘤的自动化诊断、淋巴结转移及预后评估有重要意义。更多还原

【Abstract】 Objective:Through the investigating the molecular expressions of integrinα₅β₁ in ovarian epithelial neoplasms, this study is trying to explore the relationship between the lymphatic spread of the tumor with this molecule. Method: 80 cases were examined through Elivision TM , an immune histochemistry method, for the expression of integrinα₅β₁ in ovarian epithelial neoplasms. The system of image analysis was used to measure the expression of this molecule quantitatively. Results:①Qualitative and semi-quantitative results: The expression levels of integrinα₅β₁ in the ovarian epithelial neoplasms have downward tendency in the order of benign, boundary and malignant neoplasms and there is significant difference on the expression levels of integrinα₅β₁ （ P <0.01）. There is significant difference on the expression levels of integrinα₅β₁ between the group with metastases and the group without metastases（ P <0.01）.②Quantitative examination result: The total positive cell area of the integrinα₅β₁ expression in the tumor has downward tendency in the order of benign, boundary and malignant cases, and the gray degree has upward tendency. Conclusions: The molecular expressions of integrinα₅β₁ is related to the tumor invasion and its metastases. Decreasing or lack of the expression of integrinα₅β₁ suggest the rapid development, high degree of malignancy and poor prognosis; The study also shows the improtance of using the computer image analysis for automatic diagnosis of the ovarian epithelial neoplasms,lymphatic metastases and its prognostic estimation.更多还原