【摘要】 利用改进的提取沙拐枣Calligonum mongolicumDNA的CTAB方法,从沙拐枣当年鲜根提取总DNA,以琼脂糖凝胶电泳和紫外分光光度计检测,并进行PCR扩增试验。结果显示,所提取的DNA片段大小在20 kb以上,OD260/OD280比值为1.880~1.900,OD260/OD230比值为2.000~2.040,DNA的浓度0.380~0.470μg/mL,DNA纯度较高,可直接用于随机扩增的DNA多态性(RAPD)标记,条带清晰、迁移率低而整齐。更多还原

【Abstract】 DNA was extracted from fresh root of Calligonum mongolicum using an improved method of CTAB,and examined by agarose gel electrophoresis,UV-spectrophotometer and PCR.The results showed that DNA fragment extracted by this method was larger than 20 kb,and ratios of OD₂₆₀/OD₂₈₀ and OD₂₆₀/OD₂₃₀ were 1.880～1.900 and 2.000～2.040,respectively.The concentrations of DNA were 0.380～0.470 μg/mL.The higher purity DNA can be directly used in RAPD with clear bands and low transferring rate.更多还原