【摘要】 本文采用MTT法研究了EGCG以及EGCG与锌离子相互作用对前列腺癌细胞PC-3生长的影响;采用原子吸收光谱法(AAS)研究了锌离子及EGCG共存对PC-3细胞锌、镉离子吸收的影响;利用HPLC测定了锌离子对EGCG在PBS缓冲液与正辛醇,水与正辛醇体系中分配系数的影响。结果表明,EGCG能抑制前列腺癌细胞生长,细胞存活率呈时间和剂量依赖性;锌离子能抑制PC-3细胞的生长,促进EGCG对PC-3细胞生长的抑制作用,呈时间和剂量依赖性;同时,锌离子能抑制PC-3细胞对镉离子的吸收,而EGCG有增强效应。更多还原

【Abstract】 In the present paper,effects of EGCG and interactions of EGCG with Zn2+on the growth of androgen-insensitive human prostate cancer cells(PC-3) were investigated by MTT assay.Effects of EGCG and Zn2+ on absorption of zinc and cadmium in PC-3 cells were detected by Flame Atomic Absorption Spectrometry(AAS) assay;and also the changes of Zn2+ on the partition coefficients of EGCG in n-octanol/PBS or n-octanol / water system were determined by HPLC technique.EGCG was screened for growth inhibition of PC-3 cells in dose-and time-dependent manners.Both Zn 2+ and EGCG decreased viability of PC-3 cells in dose-and time-dependent relations,and Zn2+ ameliorated the effect of EGCG on the growth of PC-3 cells.Also,Zn2+ decreased the absorption of PC-3 cells on Cd2+,and EGCG could enhance the effect as well.更多还原