【摘要】 用ISSR引物14条、RAPD引物20条,对福鼎大白茶及其回交1代94个单株进行ISSR和RAPD检测,共得到分离标记174个,其中符合孟德尔1:1分离比例的标记为90个,占标记总数的51.7%,其中ISSR标记63个,RAPD标记27个;符合3:1和1:3分离比例的标记36个,占标记总数的20.7%。利用Mapmaker 3.0软件将符合1:1、3:1和1:3分离比例的126个标记构建遗传连锁图,其中62个分子标记被归纳到7个连锁群,另外64个标记由于与该7个连锁群的距离过大而未被包含在内。在被包含在7个遗传连锁群内的62个分子标记中,有46个RAPD标记和16个ISSR标记。该遗传连锁图的总图距为1180.9 cM,标记间的平均距离为20.1 cM。其中连锁群LG4覆盖的遗传距离最大,为309.3 cM;LG6包含的标记数最多,共18个标记,平均距离15.7 cM。更多还原

【Abstract】 The segregation mode for 94 plants in first generation of back cross(BC1) of tea cultivar Fudingdabaicha were investigated using 14 RAPD primers and 20 ISSR primers.One hundred and seventy four segregation loci was identified.Among the 174 loci,90 loci or 51.7% were segregated at the expected Mendelian ratio of 1:1,in which 63 belonged to ISSR loci and 27 belonged to RAPD loci,and 36 loci segregated in the ratios of 3:1 or 1:3.Sixty-two of the 126 loci with segregation ratios of 1:1,3:1 and 1:3 were included in 7 linkage groups through linkage mapping by Mapmaker EXP 3.0 software and the other 64 loci were not included in the 7 linkage groups because of their far genetic distance.This map concluded 46 RAPD markers and 16 ISSR markers,covering distance of 1180.9 cM,with average distance of 20.1 cM.The linkage group LG4 covered the farthest genetic distance with 309.3 cM and group LG6 had the largest number of markers,with 18 loci and its average distance being 15.7 cM.更多还原