【摘要】 将DL-茶氨酸乙酰化为N-乙酰-DL-茶氨酸,利用本实验室筛选的有较高氨基酰化酶活性的真菌刺孢小克银汉霉9980(Cunnighamella echinulata 9980)对DL-茶氨酸进行拆分并对拆分条件进行摸索。结果表明:反应最适温度50℃,最适pH7.0,底物浓度0.5mol/L,湿菌体量4g/100ml,拆分时间30h,拆分率可达92%。产物经JK008阳离子交换树脂氨性柱分离,L-茶氨酸收率84.3%,[α]2D5=8.1(c=2,H2O),符合JP2000药典。更多还原

【Abstract】 An enzymatic method for the optical resolution of DL-theanine by aminoacylases from Cunnighamella echinulata 9980 was developed.The optimum temperature,pH and substrate concentration were: 50℃,pH7.0,and 0.5mol/L N-Ac-DL-theanine.The conversion rate against N-Ac-L-theanine was 92%.Productions were purified on JK008 ion exchange resin.The yield of L-theanine was 84.3%,[α]2D5 =8.1(c=2,H2O),complied with JP2000。更多还原