【摘要】 在建立茶树基因表达绝对定量实时PCR检测方法后,检测了龙井43新梢不同部位叶片中的β-葡萄糖苷酶和β-樱草糖苷酶基因的表达情况,结果表明β-葡萄糖苷酶在一芽五叶新梢的第四叶中表达活性最高,为2.86E+08拷贝/μl,第四叶>第三叶>第五叶>第二叶>一芽一叶;而β-樱草糖苷酶基因在一芽一叶中最高,为4.31E+06拷贝/μl,一芽一叶>第二叶>第三叶>第四叶>第五叶。与茶叶香气密切相关两个基因在茶树不同部位的叶片中表达量和表达类型存在明显差异。本实验建立的实时荧光定量PCR可有效地用于茶树基因表达的定量分析。更多还原

【Abstract】 β-glucosidase and β-primeverosidase are considered the most important genes related to made tea aroma.An absolute quantification method for gene expression in tea plant using real-time PCR analysis was established and,the expression of β-glucosidase and β-primeverosidase genes in different leaves of Longjing 43 young shoot were determined using this method.The results indicate that the fourth leaf of the ‘five and a bud’ shoot has the highest copy number of β-glucosidase mRNA,which is 2.86E+08 copies/μl,the 4th leaf>the 3rd leaf>the 5th leaf>the 2nd leaf>one and a bud.And the highest β-primeverosidase expression is in the one and a bud,corresponding to 4.31E+06 copies/μl,one and a bud>the 2nd leaf>the 3rd>the 4th leaf> the 5th leaf.The expression intensity and pattern of these genes,which are closely related to tea aroma,are different in the young shoot.The results illuminate that the new developed method could be effectively used to detect gene expression quantitatively in tea plant.更多还原