【摘要】 为探讨利用鳞翅目昆虫对环境激素进行生物学评价和监测的方法,用2,3-二苯基溴化四唑（MTT）、4’,6二乙酰基-2-苯基吲哚（DAPI）荧光染色和单细胞凝胶电泳（SCGE）、流色细胞仪检测（FCM）等技术,分析检测了环境激素阿特拉津（AT）对体外培养家蚕卵巢细胞（BmN）细胞增殖和凋亡的影响。结果显示：AT浓度超过0．0625mmol/L,显著抑制了BmN培养细胞48h的存活率（p＜0．001）,LC₅₀为0．0703mmol/L；0．0625～0．5000mmol/L AT处理24h后,BmN细胞核出现明显的凋亡现象；0．5000mmol/L的AT处理48h后,BmN细胞DNA链发生断裂。AT对BmN细胞增殖和凋亡的影响有明显的时间-剂量效应。长期处于AT污染的环境中对鳞翅目昆虫可能有严重的细胞毒性。更多还原

【Abstract】 In order to explore the biological methods by using lepidopterous insect to evaluate and monitor en- vironmental hormone,the inhibitory effect of atrazine（AT）on the cell growth and apoptosis of ovary cells of Bombyx silkworm（BmN）in vitro was measured by MTT[3-（4,5）dimethylthiazol-2,5-diphenyl-tetrazolium bro- mide],SCGE（single-cell gel electrophoresis）,DAPI（4’,6-diamidino-2-phenylindole）and FCM（flow cytome- ter）assays.Results showed when the concentration of AT is over 0.0625mmol/L,it inhibits BmN cell survival rate of 48h remarkably（p＜0.001）,and the LC₅₀ is 0.0703 mmol/L.After 24 hours,the nucleolus of cells treated by 0.0625～0.5000mmol/L AT appeared apoptosis clearly.AT can induce the breakage of DNA af- ter 48 hours cultured with 0.5000mmol/L.AT has remarkable time-dosage effects on the cell growth and ap- optosis.The results suggested that living in the condition polluted by AT for a long time,cells of lepidopterous insect be poisoned badly.更多还原