【摘要】 基于DNA载体的RNA i技术可以较长时间高效地抑制基因的活性。利用家蚕核型多角体病毒ie-1基因启动子,构建了在细胞内能转录形成与ie-1基因+19~+446区域互补同源的发夹状dsRNA的RNA i DNA载体。研究结果表明,该载体DNA在体外、体内均能较好地抑制家蚕核型多角体病毒在细胞中的复制。更多还原

【Abstract】 RNAi based on DNA vector is able to inhibit the activity of genes effectively in a longer period.With the ie-1 promotor of Bombyx mori nucleopolyhydrovirus,the DNA vector of RNAi was constructed,which can transcribe dsRNA in the cells to form hairpins and complementary to the region from +19～+446 of ie-1 gene of BmNPV.Experiments demonstrated that the DNA vector inhibited the replication of BmNPV both in vivo and in vitro.更多还原