【摘要】 目的建立体外培养星形细胞牵张损伤后继发低氧二次损伤动物模型。方法用计算机控制,以不同压力牵张损伤培养于硅胶膜培养皿上的大鼠星形细胞,并于牵张损伤后立即予以1小时低氧应激,用乳酸脱氢酶(LDH)和台盼蓝定量检测星形细胞在不同条件下和不同时相点的损伤程度。结果不同程度的牵张压力可导致不同程度的细胞损伤,通过控制牵张压力可以调控损伤的程度;LDH与台盼蓝检测的结果明显相关;动态观察结果显示LDH的释放高峰主要在1小时之内;单纯短暂低氧应激后星形细胞LDH释放无明显升高,与正常对照组相比无显著性差异(P>0.05)。但牵张损伤后复合低氧应激后,LDH释放量明显升高,与单纯牵张损伤组相比较,有显著性差异(P<0.01)。结论创伤后低氧可显著增加体外培养星形细胞的损伤程度,此模型可用于体外研究创伤后低氧二次打击的机制。更多还原

【Abstract】 Objective To develop a model of traumatic injury with secondary hypoxia insult on cultured rats astrocytes in vitro.Methods Rat cortical astrocytes cultured in tissue culture wells with a flexible silastic bottom were injured by stretch device.The pressure of the stretch device can be controlled and on line tested by the computer.After stretch-induced injury,the astrocytes were insulted by 1h hypoxia stress.Extent of cell injury was qualitatively assessed by lactate dehydrogenase(LDH) enzyme release and trypan blue staining.Results The diferent degree of cell injury was caused by different gas pressure.The degree of cell injury might be controlled by the different pressure.There was some significant correlation between the measured value of LDH and trypan blue staining.It was shown that the peak of LDH release is at 1h.The LDH release of astrocytes was not significantly increased after only hypoxia stress(P>0.05),but the secondary hypoxia stress after stretch-induced injury caused significant increase of LDH release of cultured astrocytes as compared with stretch-induced astrocyte(P<0.01).Conclusion Traumatic injury with secondary hypoxia insult can significantly increase the degree of injury of cultured astrocyte in vitro.The model can be used to study the mechanism of traumatic injury with secondary hypoxia insult in vitro.更多还原