【摘要】 目的:建立为临床用药提供血药浓度检测方法。方法:建立用高效液相色谱法测定奥曲肽(别名生长抑素,商品名善宁)的方法,血样经过超滤后,调pH为4,在大孔径300 Octreotide的Krom asil C18柱(4.6 mm×250mm,5μm)上以乙腈∶水∶三氟乙酸(体积比20%∶80%∶0.01%)为流动相,经二极管阵列检测器检测。结果:善宁的回收率为85%。在浓度0.006~0.5 mg/mL有良好的线性关系。结论:可采用大孔径液相色谱柱检测溶液和善宁的含量。更多还原

【Abstract】 Objective: To develop a method in the determination of somatostatin level in blood for clinical medication. Methods: A method for the determination of somatostatin(Octreotide) in serum by high performance liquid chromatography with diode-array detector(DAD) was developed.After ultrafiltration,the pH values of blood samples were adjusted to 4,and samples were analyzed by Kromasil C18 column(4.6 mm× 250 mm,5 μm i.d.) with a large pore(300) by acetonitrile/water/trifluoroacetic acid(20/80/0.01%,v/v) as mobile phase. Results:The recovery rate of Octreotide was 85%,and the calibration curve showed good linearity in the range of 0.006-0.5 mg/mL. Conclusion: The method is suitable for the detection of Octreotide content by liquid chromatography.更多还原