【摘要】 目的:研究肿瘤坏死因子(TNFα-)对谷氨酰胺(G ln)调控大鼠肝蛋白质合成的影响。方法:将30只雄性SD大鼠,随机分为A(TPN)组;B(TPN+G ln)组;C(TPN+G ln+TNFα-)组,均行72 h全肠外营养。B组加用丙氨酰谷氨酰胺二肽,其中G ln剂量为0.3 g/(kg.d),计72 h。C组在B组的基础上于实验结束前24 h持续静脉滴注TNFα-,速度为5μg/(kg.h)。所有大鼠均在实验结束前0.5 h,一次性静脉注射L-15N亮氨酸1.0 mmol/kg。实验结束时,分别测定血浆中TNFα-浓度,血浆及组织中G ln浓度,并测定肝组织的蛋白质合成率。结果:B组血浆及肝组织中G ln浓度高于A组,但两组均低于C组;B组肝蛋白质合成率高于A组,C组低于B组。结论:TNFα-能升高血浆及肝组织中G ln的浓度;G ln能促进肝蛋白质合成;而G ln这种促肝蛋白质合成作用可被TNFα-抑制。更多还原

【Abstract】 Objectives:The study was desigued to observe the regulations of glutamine metabolism by TNF-α. Methods:Thirty male SD rats were assigned into three groups: A(TPN),B(TPN+Gln),C(TPN+ Gln+TNF-α).At the last 0.5 hour,all animals were maintained with \ leucine.Plasma TNF-α levels concentrations of glutamine plasma and in tissues were measured as the experiment ended after 72 hours,and the fractional synthesis rates(FSR) were also assessed.Results:The glutamine concentrations of plasma and liver were increased markedly in group B and C.FSR was increased in group B and decreased in group C.Conclusions:The results indicated that TNF-α could increase the levels of glutamine in plasma and tissues and attenuate promoting protein synthesis of glutamine in tissues.更多还原