【摘要】 目的:利用绿色荧光蛋白(GFP)标记技术明确化疗时是否存在细菌移位。方法:将30只SD大鼠随机分为三组:空白对照组、对照组和甲氨蝶呤(MTX)组,每组10只。给对照组和MTX组大鼠灌饲GFP标记的大肠杆菌TG1对移位的细菌进行示踪,MTX组大鼠皮下注射MTX制作大鼠化疗模型。使用荧光显微镜及质粒酶切电泳鉴定内脏分离出的细菌是否来源于肠道。结果:在应用MTX大鼠的肠系膜淋巴结、肝、脾和肾,均可分离出GFP标记E.coliTG1。结论:MTX能引起细菌移位,在细菌移位的动物实验研究中,GFP示踪技术是一种简单、可靠和有效的方法。更多还原

【Abstract】 Objective: To confirm the bacterial translocation induced by Methotrexate(MTX).Methods: ThirtySprague-Dawley(SD)rats were randomly divided into threegroups: blank group(n=10),control group(n=10),MTX group(n=10).The ratsof control groupandMTX groupweregavaged EscherichiaColi(E.coli)TG1 labeledwith green fluorescent protein(GFP) to track bacterial translocation.The ratsof MTX groupwere treated with Methotrexate to induce bacterial translocation.Representative tissue specimens from the mesenteric lymph nodes(MLNs),spleen,liver and kidenywere aseptically harvested forbacteria culture in ampicillin-supplemented medium.Results: Thebacteria labeled withgreen fluorescent proteinwere detected in tissue specimens harvested from rats treated with MTX,but were not detected in the other groups.Conclusion: MTXcan induce intestinal bacterial translocation in rats.GFP is a stable,simple,useful marker for investigation of intestinal bacterial translocation in animals.更多还原