【摘要】 以赤子爱胜蚓(Eisenia foetida)为材料,λTriplex2TM为载体,利用SMART技术构建全长cDNA文库,从而筛选全长目的基因并研究其结构和功能。经测定原始文库滴度为1.3×106pfu/mL,扩增总文库滴度为1.0×108pfu/mL,重组率达到99%以上,插入片段长度在1.1 kb左右。该文库的建立为功能性新基因的发现奠定了坚实的基础。更多还原

【Abstract】 To obtain a full-length cDNA library of Eisenia foetida for the purpose of gene screening and functioning study,total RNA extraction method from Eisenia foetida used is TRIZOL Reagent to construct a cDNA library by the carrier of λTriplex2^TM using the SMART cDNA Library Construction Kit.The quality of the constructed full-length cDNA library was evaluated.The primary library showed a titer of 1.3×10⁶ pfu/mL of the cDNA insets,and the amplified library has a titer of 1.0×10⁸.The recombinant rates were about 99% and the average length of the cDNA inserts was about 1.1 kb.This constructed cDNA full-length library provided an essential resource for the further gene functional studies and gene discoveries of Eisenia foetida.更多还原