【摘要】 蛋白质组学霰弹法策略是大规模分析复杂蛋白质混合物的有力方法,它以多维色谱和串联质谱为核心技术,随着数据库和计算机分析数据的能力而发展。本文综述了它的实验流程、MS/MS(tandemmassspectrometry,MS/MS)数据解释的方法、过程和解释中存在的问题以及在实际研究中的应用。更多还原

【Abstract】 Shotgun strategy for proteomics were as a powerful approach for high-throughput analysis of complex protein mixtures. Based on the integration of multi-dimensional chromotograph and mass spectrometry and evolving with the proteomic informatics, its experimental protocol, interpretation of MS/MS data and application were reviewed and discussed.更多还原