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苦参碱与6种抗肿瘤药联用对KBV200耐药细胞株P-糖蛋白表达的研究
Investigation of the effect of combined matrine and chemotherapeutic agents on P-gp of KBV200 cell line
【摘要】 目的探讨6种常用抗肿瘤药物长春新碱、阿霉素、平阳霉素、顺铂、5-Fu、环磷酰胺等与苦参碱联合对KBV200耐药细胞株P-糖蛋白(P-gp)表达的影响。方法以喉癌KBV200耐药细胞株为研究对象,通过流式细胞仪(FCM)检测6种抗肿瘤药物作用于KBV200耐药细胞株后P-pg表达以及分别联合苦参碱后对P-pg表达的影响。结果苦参碱分别联合长春新碱、阿霉素作用后,KBV200耐药细胞株P-pg表达水平下降。结论苦参碱分别与长春新碱和阿霉素联合作用后,可使KBV200耐药细胞株P-pg的表达水平下降,从而逆转其耐药性。
【Abstract】 Objective To observe the effect of matrine in combination with 6 chemotherapeutic agents vincristine,adriamycin,bleomycin,cyclophospharmide,5-Fu,cisplatin on the expression of P-glycoprotein(P-gp) of KBV200 cell line.Methods Flowcytometer(FCM) assay was used to investigate the expression of P-gp and the effect on expression of KBV200 cell.Results Matrine in combination with vincristine(VCR) and adriamycin can reverse the multidrug resistance(MDR) by decreasing the expression of P-gp.Conclusion Matrine in combination with vincristine and adriamycin can cut down the expression of P-gp of the KBV200 cell line.
【Key words】 KBV200 multidrug resistance cell line； MDR； reversion of multidrug resistance； P-gp； matrine；
- 【文献出处】 重庆医学 ,Chongqing Medicine , 编辑部邮箱 ,2006年14期
- 【分类号】R739.65
- 【被引频次】15
- 【下载频次】236