【摘要】 目的探讨高脂饮食诱导的胰岛素抵抗大鼠糖代谢及抵抗素、脂联素水平的变化。方法采用大鼠扩展胰岛素钳夹和3-3H标记葡萄糖示踪技术,估计高脂诱导胰岛素抵抗(IR)大鼠肝糖及外周组织糖代谢的变化,同时采用酶免法检测血浆抵抗素及脂联素水平。结果高脂喂养组(HF组)大鼠基础血浆游离脂肪酸(FFA)水平明显增高。钳夹稳态时,HF组血浆胰岛素水平明显高于N组,差异有统计学意义(P<0.01),FFA也明显高于N组,差异有统计学意义(P<0.01)。HF组葡萄糖输注率(GIR)较N组减少38%,胰岛素介导的葡萄糖利用率(GRd)较N组亦明显减少21%。N组大鼠肝糖输出(HGP)明显抑制达71%,而在HF组胰岛素介导的HGP的抑制作用受到明显障碍(仅抑制30%)。钳夹结束时,N组血浆抵抗素水平与基础值相比明显降低,差异有统计学意义(P<0.05),而HF组明显高于N组(P<0.05)。两组血浆脂联素水平在钳夹后均明显下降差异有统计学意义(P<0.05)。结论高脂饮食诱导大鼠产生了外周和肝脏的IR。IR的形成可能与血浆FFA升高和在高胰岛素状态下较高的血浆抵抗素水平有关。更多还原

【Abstract】 Objective To investigate the changes of glucose metabolism,plasma resistin and adiponectin levels in insulin-resistant rats induced by high-fat diet.Methods Changes of peripheral and hepatic glucose metabolism in awake rats were evaluated by hyperinsulinemic euglycemic clamp technique combined with 3-3H glucose as a tracer.And plasma resistin and adiponectin were detected by ELISA.Results In baseline,plasma free fatty acids(FFA) levels were increased in high-fat diet rats(HF) compared with normal chow diet rats(N).During clamp steady-state,plasma insulin and FFA levels were higher in HF group compared with control(P<0.01).In HF group,the glucose infusion rate(GIR) and glucose disappearance rate(GRd) were reduced by 38% and 21%,respectively.And the insulin-mediated suppression of hepatic glucose production was up to 71% in N group,but it was impaired and only up to 30% in HF rats(P<0.01).At the end of the clamp,plasma resistin levels were decreased in N group and lower than that in HF rats(P<0.05).Howevre,plasma adiponectin levels were both decreased after clamp(P<0.05).Conclusion High-fat diet induced peripheral and hepatic insulin resistance in rats.It might be associated with elevated FFA and higher plasma resistin levels during hyperinsulinemia.更多还原