【摘要】 目的探讨肥胖与内皮损伤的关系及其相关因素。方法采用化学比色法、放免法和RT-PCR法分别测定40例单纯超重和肥胖组与25例正常对照组的血浆NO、ET-1水平和单个核细胞MCP-1表达水平的变化。同时测定FPG、BMI、WC、WHC、TC、TG、HDL、LDLI、RI、HBCI等临床指标。结果单纯超重和肥胖组的NO显著降低,ET-1和MCP-1显著升高(均为P<0.000 1),逐步回归分析示BMI、TCI、RI对血浆NO有影响(标准偏回归系数分别为-0.5754-、2.960 9-、0.204 9);BMI、HDL、TCI、RI对血浆ET-1有影响(标准偏回归系数分别为0.35、-0.25、0.41、0.20);HDL、TC、WC对MCP-1表达有影响(标准偏回归系数分别为-0.03、0.96、0.03)。结论肥胖时脂代谢紊乱、IR、肥胖程度、脂肪分布异常均是引起内皮损伤的独立因素,且脂代谢中的胆固醇升高是首要因素。更多还原

【Abstract】 Objective To investigate the relationship between obesity and endothelial damage and the related factors in the process.Methods To measure the change of NO,ET-1 and MCP-1 in the plasma of single overweight and obesity group(40 cases) and normal control group(25 cases) by RIA and RT-PCR.Results The NO level obviously decreased and ET-1and MCP-1 sufficiently increased(all P<0.0001) in single overweight and obesity group.Stepwise regression indicated that BMI,TC,IRI had influence on NO(standard partial regression coefficient were-0.575 4,-2.960 9,-0.204 9 respectively),BMI HDL TC and IRI had effect on ET-1(standard partial regression coefficient were 0.35,-0.25,0.41,0.20 respectively);HDL,TC,WC worked on the expression of MCP-1(standard partial regression coefficient were-0.03,0.96,0.03 respectively).Conclusion In obesity,the lipid decompensation,IR,the degree of obesity and distribution of fat are all independent factors attributed to cause endothelial damage,moreover the increased cholesterol is the most important factor.更多还原