【摘要】 目的永久性结扎成年大鼠双侧颈总动脉导致慢性颈动脉系统灌注不足(CVI),分析其行为学及病理学改变。方法Wistar大鼠110只分为假手术组和单纯缺血组。单纯缺血组永久性结扎成年大鼠双侧颈总动脉建立大鼠CVI模型。于术后第3、9周行Morris水迷宫测试分析CVI大鼠术后视空间记忆损害的特点;采用HE、Bielschowsky银浸染法及免疫组织化学分析术后不同时间实验动物脑组织病理改变。结果各组大鼠均无明显运动功能障碍。单纯缺血组大鼠第3周平台定位时间、搜索策略均较假手术组明显延长(P<0.01),第9周尤为明显(P<0.001)。病理学结果显示,单纯缺血组呈轻度缺血性改变,神经元减少代之以胶质细胞增生;银染色法示部分顶叶、海马神经细胞嗜银样变,突起迂曲,类似神经元纤维缠结样改变。结论永久性结扎成年大鼠双侧颈总动脉引起的慢性CVI模型出现行为学障碍及脑组织轻度缺血性病理学改变,提示慢性CVI可能在神经变性疾病病理过程中起一定作用。更多还原

【Abstract】 Objective To study the behavioral and pathological changes of the rats with chronic pcerebrovascular insufficiency (CVI) induced by permanent ligation of bilateral common carotid arteries. Methods Wistar rats (n=110) were divided into CVI group and sham-operation group. The CVI models were produced by permanent ligation of bilateral common carotid arteries. Morris water maze test were done at week 3 and 9 after operation for detecting the visuo-spatial memory impairment. Their pathological changes in brain were primarily studied by HE, Bielschowsky silver stainings, and immunohistochemistry for β-tubulin and GFAP. Results There was no difference in motor function evaluation between 2 groups, but the CVI group displayed significant deficits in locating the hidden platform, and in search strategy in Morris water maze at week 3 (P<0.01 vs sham-operation group), and the memory dysfunction became more serious at week 9 (P<0.001 vs sham-operation group). The histological examination showed mild to moderate ischemic changes, which included a moderate loss of hippocampal CA1 cells together with increasing GFAP immunoreaction. Some abnormal neurons in the hippocampus and cortex were manifested as dark by HE staining and argyrophilic by modified Bielschowsky silver staining, and these argyrophilic neurons showed such a similar feature as neurofibrillary tangles. Conclusion The CVI rat model made by permanent ligation of bilateral carotid arteries shows significant cognitive impairment and chronic progressing ischemic changes in brain, which indicates that chronic CVI maybe plays a role in the progression of primary neurodegenerative disease.更多还原