【摘要】 目的探讨肿瘤坏死因子-α(TNF-α)基因多态性与广东地区汉族人群散发性阿尔茨海默病(SAD)发病易感性的关系。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)方法,检测44例SAD病人(AD组)及45例正常老年人(对照组)TNF-α基因的TNF-α1及TNF-α2等位基因频率。按比值比(OR)作疾病关联分析。用放免法检测两组的血清TNF-α含量。结果AD组的TNF-α2基因频率为0．182,明显高于对照组的0．078(OR=2．635,P=0．039)；AD组血清TNF-α含量为(567．85±102．43) ng/L,明显高于对照组的(389．73±56．68)ng/L(P＜0．05)。结论广东地区汉族人群中,TNF-α基因多态性与SAD有关联．TNF-α2等位基因与SAD的易感性有关。更多还原

【Abstract】 Objective To explore the relation between the tumor necrosis factor-α(TNF-α)gene polymorphism and the susceptibility to sporadic Alzheimer’s disease(SAD)in a Guangdong Han population.Methods The frequencies of TNF-αl and TNF-α2 alleles of TNF-αgene were detected by polymerase chain reaction-sequence specific primers(PCR-SSP)in 44 SAD cases and 45 normal elders as controls.The differences in polymorphic distribution between the two groups were tested,and odds ratio was computed for association analysis.The content of TNF-αin serum was measured by radioimmunoassay. Results The frequency of TNF-α2 allele was significantly increased in patients with SAD compared to that of the controls(0.182 vs 0.078,OR=2.635,P=0.039).The content of TNF-αin serum in SAD group was (567.85±102.43)ng/L,which was significantly higher than that(389.73±56.68)ng/L in control group(P＜0.05).Conclusion In Han population in Guangdong province,TNF-αgene polymorphism is correlated to the risk for SAD.TNF-α2 allele may play a role in SAD susceptibility,while TNF-αl allele may be a protective gene against SAD.更多还原