【摘要】 目的探讨BKCa通道对细胞蛋白酪氨酸磷酸化水平的调节作用。方法观察转染 BKCa通道及共转染BKCa通道与v-Src对HEK293细胞蛋白酪氨酸磷酸化水平的影响。比较BKCa通道激动剂NS1619和阻断剂paxilline对培养海马神经元蛋白酪氨酸磷酸化水平的影响。结果 HEK293细胞表达BKCa通道后细胞蛋白酪氨酸磷酸化水平降低,同时BKCa通道能够降低转染v-Src 后的细胞蛋白酪氨酸磷酸化水平。BKCa通道激动剂NS1619能够降低海马神经元蛋白酪氨酸磷酸化水平,阻断剂paxilline能增加细胞蛋白酪氨酸磷酸化水平。结论 BKCa通道的表达可以降低细胞蛋白酪氨酸磷酸化水平。更多还原

【Abstract】 Objective To study the regulation of cellular protein tyrosine phosphorylation by large conductance calcium-activated potassium (BKCa) channels. Methods The change of protein tyrosine phosphorylation was detected after transfection of BKCa, channels or cotransfection of BKCa channels and v-Src in HEK293 cells. The effects of BKCa channel agonist NS1619 and antagonist paxilline were compared on the protein tyrosine phosphorylation of hippocampal neurons in vitro. Results Protein tyrosine phosphorylation by endogenous and expressed v-Src tyrosine kinase was reduced markedly by the expression of BKCa channels in HEK293 cells. The cellular protein tyrosine phosphorylation of hippocampal neurons was decreased by BKq, channels agonist NS1619, but increased by BKq, channel antagonist paxilline. Conclusion Expression of BKCa channels decreases cellular protein tyrosine phosphorylation.更多还原