【摘要】 采用5种酶对脱脂鲢肉进行水解制备水解鲢蛋白。脱脂鲢肉是分别采用异丙醇、正己烷、乙酸乙酯3种溶剂萃取法进行脱脂后得到。分别用木瓜蛋白酶、枯草杆菌中性蛋白酶、枯草杆菌碱性蛋白酶、胰蛋白酶和复合蛋白酶对3种脱脂鲢肉及未脱脂鲢肉进行水解。试验结果表明,乙酸乙酯对鲢肉脱脂率最高,脱脂率为96.33%。复合蛋白酶为最适酶,乙酸乙酯脱脂鱼肉为最佳底物;在最佳水解条件下:pH 6.5,温度55℃,酶量3500 U/g蛋白质,底物浓度(乙酸乙酯脱脂鲢肉∶水)1∶5,其水解度达到最大,为44.00%。更多还原

【Abstract】 The degreasing methods for silver carp(Hypophthalmichthys molitrix) flesh and enzymatic hydrolysis of defatted meat were investigated.The mximum degreasing rate(96.33%) was obtained by acetidin extraction.The defatted meat after solvent extraction was hydrolysed using papain,neutral protease,alkaline protease,trypsase and protamex protease,respectively.The results showed that the maximum hydrolysis degree(DH) of(44.00%) was found to be produced by protamex under the optimal hydrolysis conditions: pH 6.5,55 ℃,3500 U enzyme /g protein and a ratio of enzyme to substrate 1∶5.更多还原