【摘要】 目的:测定赤芍在不同土壤肥力条件中芍药苷的含量。方法:采用高效液相色谱法,Spherisorb 10 ODS1(4.6 mm×250 mm,10μm),以甲醇∶0.05%mol/L磷酸二氢钾溶液(40∶65)为流动相,检测波长为230 nm。线性范围2.5~12.5μg,相关系数为0.99998。平均回收率为100.5%,RSD=2.2%(n=5)。结果:芍药苷在不同土壤条件下的平均含量分别为9.01%、5.56%、9.69%、8.23%、9.33%、6.22%、7.20%。结论:该方法准确,快速,重现性好,可作为赤芍栽培中内在质量评价的重要依据。更多还原

【Abstract】 Objective:To compare the contents of paeoniflorin in the different fertility of soil of paeonia lactiflora.Method:The contents of paeoniflorin determined by HPLC.Chromatographic condition included Spherisorb 10 ODS1（4.6 mm×250 mm,10?μm） and the mobile phase consisting of a mixture of methanol-0.05%mol/LKH₂PO₃（40∶65）.Paeoniflorin was detected at 230 nm.The standard curve was linear in the range of 2.5～12.5?μg with correlation coefficient 0.99998.The average recovery of paeoniflorin was 100.5% with RSD=2.2%.Results:The contents of paeoniflorin were 9.01%,5.56%,9.69%,8.23 %,9.33%,6.22%,7.20% in the different fertility of soil respectively.Conclusion:The method is accurate quick and good reappearance,it can be used as the important basis of quality estimation in the culture of paeonia lactiflora.更多还原