【摘要】 目的:检测分析版纳微型猪近交系基因组中内源性逆转录病毒(porc ine endogenous retrovirus,PERV)序列的拷贝数,从而为猪源移植物的异种移植筛选理想供者。方法:用PERV的pol,env各亚型基因及envA/C重组体的特异性引物,对4个近交系亚系共50头以及5头欧洲大白猪的外周血单核细胞(peripheral b lood mononuc learcell,PBMC)的基因组DNA进行了实时定量/半定量PCR检测及普通PCR检测。结果:所有版纳猪基因组中可特异扩增出pol,envA,envB片段,其平均拷贝数分别为(24.2±9.4),(13.1±8.4)和(16.4±9.8)个,但近交系不同亚系之间各片段扩增产物量比较差异具有统计学意义(P<0.01)。所有样品中无法扩增出envC片段以及envA/C重组体。结论:版纳微型猪近交系基因组内,PERV的envA、envB和pol序列拷贝数在近交系不同亚系间比较差异有统计学意义,不存在envC和envA/C重组体序列。更多还原

【Abstract】 Objective: To investigate the copy numbers of porcine endogenous retroviruses in genome of Banna miniature swines for screening of donors in xenotransplantation with porcine transplants.Methods: The genomic DNA of peripheral blood mononuclear cells(PBMCs) of 50 Banna miniature swines in 4 inbred sublines and 5 European Large White Pigs was extracted for the detection to pol,envA,envB,envC and envA/C recombinant in PERVs with real-time quantitative/ semi-quantitative PCR and normal PCR.Results: PCR products of pol,envA and envB of PERV could be obtained from all the Banna samples,and the mean amount of these products was 24.2±9.4,13.1±8.4,16.4±9.8 respectively and was significantly different among the inbred herds(P<0.01).The envC and envA/C were not amplified.Conclusion: The copy numbers of pol,envA and envB genes of PERV in porcine genome were significantly different among the inbred herds of Banna miniature swines,and envC and envA/C were absent in genome of Banna Miniature Swines.更多还原