【摘要】 目的:通过分析肝郁证模型大鼠血清蛋白质组的差异表达,从蛋白质组学角度探讨肝郁证的实质。方法:以束缚制动法制备肝郁证大鼠模型,二维凝胶电泳(2-DE)分离大鼠血清蛋白质,获得差异表达蛋白质;利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)和数据库分析鉴定差异表达蛋白质。结果:发现9个具有明显表达差异的蛋白质斑点,经鉴定可以确定的6个蛋白质分别为:转甲状腺素蛋白(transthyretin,TTR)、水解酶Ba1-647、血清白蛋白前体、抗乙酰胆碱受体抗体Fab片段、Ig-λ2链C区和β1球蛋白。结论:筛选出的差异表达蛋白质主要涉及免疫、神经内分泌和营养物质代谢方面,和现有研究结果相符,证实从蛋白质组学的角度研究证实质是可行的。更多还原

【Abstract】 Objective: To explore the essence of stagnation of liver-QI syndrome though analyzing differential expression of serum proteome on stagnation on liver-QI syndrome model rats.Methods: Liver-QI syndrome model rats were prepared by restraints.Serum protein was separated by 2D gel electrophoresis（2-DE）,so as to gain differential expression protein.Differential expression protein was identified by MALDI-TOF-MS and database analysis.Results: 9 obviously differential expression protein spots were found.Though identification,6 proteins could be definite in terms of transthyretin（TTR）,hydratase Ba1-647,seralbumin precursor,anti-acetylcholine receptor antibodies Fab fragment,Igλ-2 chain C section and β₁ globulose.Conclusion: Differential expression proteins selected refer to immune,neuroendocrine and nutrient substance metabolism,correlated with modern findings,so it can be confirm that it is feasible to research syndrome essence from proteome logy.更多还原