【摘要】 目的:观察抗纤Ⅰ号(AF)和抗纤Ⅰ号加硒(AS)药物血清诱导大鼠肝星状细胞(HSCs)增殖、凋亡的作用,并探讨其作用的分子机制。方法:采用整体动物体内给药,分离制备含药血清,采用MTT法检测药物血清对HSCs增殖的影响;流式细胞仪检测细胞周期;Annexin-V/PI联合标记法测定细胞凋亡率;RT-PCR测定HSCs转化生长因子β1(TGF-β1)mRNA表达;电泳迁移率改变分析(EMSA)检测核转录因子κB(NF-κB)与DNA的结合活性。结果:AF和AS含药血清呈剂量依赖性抑制HSCs增殖,并可诱导HSCs凋亡,凋亡率(%)分别为18.07±2.67,17.55±1.91;两种含药血清可显著降低HSCs TGF-β1mRNA表达,抑制HSCs NF-κB的结合活性。结论:抗纤Ⅰ号和硒药物血清可以通过抑制HSCs的增殖、诱导HSCs凋亡,而使细胞外基质合成减少,肝纤维化减轻,其可能的分子机制是抑制NF-κB的活性,进而降低TGF-β1的表达。更多还原

【Abstract】 Objective: To investigate the sera pharmacological effect of anti-fibrosis herbs and selenium on the proliferation and apoptosis of hepatic stellate cells.Methods: The sera from the rats fed by colchicines（Col group）,anti-fibrosis herbs（AF group） and anti-fibrosis herbs plus selenium（AS group） acted on the hepatic stellate cells（HSCs）.MTT assays and Flow Cytometry were employed to estimate the proliferation of HSCs.The expressions of TGF-β₁mRNA were detected by semi-quantified RT-PCR.The activity of NF-κB binding DNA was examined by EMSA.Results: The inhibitive rate of AF group and AS group were much higher than that of Col sera at the concentration of 10%～40%.The proliferation indexof AF group and AS group was lower than that of Col group statistically.Sera of treatment group could induce the apoptosis of HSCs.The expressions of TGF-β₁mRNA in AF and AS group were markedly decreased compared with Col group.The activity of NF-κB binding DNA of AF group,AS group was much lower than that of Col group.Conclusion: These results suggested that anti-fibrosis herbs and selenium may inhibited the proliferation of HSCs and induce apoptosis of HSCs in vitro,which may be related to down-regulation the activity of NF-κB binding DNA and TGF-β₁ expression.更多还原